MafA-positive insulin-producing cells derived from rat adipose stem cells are efficient in glucose hemostasis in vitro

Objective: MafA has been recognized as a specific pancreatic β-cells activator for Insulin expression. This study investigated the changes in glucose transport and consumption by MafA-positive Insulin-producing cells resulting from differentiation of rat adipose tissue-derived mesenchymal stem cells. Methods: Adipose were differentiated into 14-day protocol using nicotinamide ITS. The ability to produce was confirmed adipogenesis capacity expression Oct4, Nanog, Sox2. Glucagon concentrations determined ELISA. presence MafA, Glut2, ENO1, IGF-I receptor genes Western blotting analysis. amounts Glucokinase Pyruvate Carboxylase activities measured Fluorometric Colorimetric methods, respectively. Results: un-differentiated showed promising differentiate toward adipocytes. tissue revealed significantly elevated levels Sox2 compared that secreted considerably more than undifferentiated